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Do I need a tune to run E85 on an otherwise stock LS?

Yes. Performance Racing Industry's points on E85:

  • More fuel: E85 needs about 30–40% more fuel flow to hit the same air-fuel ratio, so the tune must add fuel, and the injectors and pump must keep up (injectors at or below 90% duty cycle, pump with 10–20% headroom).
  • Cold starts need their own adjustment; ethanol cold starts can be hard.
  • Blend varies: pump "E85" can be as low as 51% ethanol, so a tune for one fixed blend is vulnerable to variation.
  • Flex fuel: an ethanol-content sensor lets the computer adapt to whatever blend is in the tank, and also run on gasoline.

See fuel, E85 & boost.

Want to do it yourself? See who tunes it, and how to learn.

Confidence: medium — sources disagree or are limited; check the sources below.

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Sources
  1. PRI Education: Converting From Pump Gas to E85 — Performance Racing Industry